Table of Contents
Gas Chromatography
Gas Chromatography
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Principles
Key Information
Gas Chromatography
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Schematic of a gas Chromatograph
The Beginning
Today
Uses of Gas Chromatography
Instrumentation
Instrumentation
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GC detectors another powerpoint
Liquid Chromatography much slower diffusion in liquid as compared to gas
Liquid liquid extraction repeated extraction is basis for LC
Retardation of solutes in liquid onto a solid phase
Elution chromatography
Types of Liquid Chromatography
Types of Liquid Chromatography
Retardation based on size of molecule as it diffuses into porous solid
High Performance Liquid Chromatography
Outline
What is HPLC?
HPLC is….
History lesson
Advantages to HPLC
Advantages to HPLC
Liquid chromatography
Schematic of liquid chromatograph
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Types of HPLC
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Partition Chromatography
Partition Chromatography II
Partition Chromatography III
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Adsorption Chromatography
What is Ion Chromatography?
The Mobile Phases are...
Properties of the Mobile Phase
Ion-Exchange Packings
Ion-Exchange Equilibria
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Eluent Suppressor Technique
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Size Exclusion Chromatography(SEC)
SEC(continued)
SEC Column Packing
Advantages of Size Exclusion Chromatography
Disadvantages
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Instrumentation
Schematic of liquid chromatograph
Mobile phase reservoir
Elution methods
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Pumping System I
Pumping System II
Pumping System III
Sample Injection Systems
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Liquid Chromatographic Column
Liquid Chromatographic Columns II
Detector
Some Properties of Detector
More Properties of Detector
Types of Detector
Refractive Index I
Refractive Index II
Refractive Index III
UV/Visible I
UV/Visible II
UV/Visible III
UV/Visible IV
Fluorescence I
Fluorescence II
Conductivity
Evaporative Light Scattering I
Evaporative Light Scattering II
Electrochemical I
Electrochemical II
Data System
Electrophoresis…charged species migrate in electric field Separation based on charge or mobility
Capillary electrophoresis higher voltages can be used as the heat can be dissipated
Capillary electrophoresis
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